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KMID : 0368420160590050496
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Journal of Plant Biology
2016 Volume.59 No. 5 p.496 ~ p.505
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Map-based cloning and functional analysis of the chromogen gene C in rice (Oryza sativa L.)
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Zhao Shasha
Wang Cuihong
Ma Jian
Wang Shuai
Tian Peng
Wang Jiulin
Cheng Zhijun
Zhang Xin
Guo Xiuping
Lei Cailin
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Abstract
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The chromogen gene C is critical for anthocyanin regulation in rice, and apiculus color is an important agronomic trait in selective breeding and variety purification. Mapbased cloning and in-depth functional analysis of the C gene will be useful for understanding the molecular mechanism of anthocyanin biosynthesis and for rice breeding. Japonica landrace Lijiangxintuanheigu (LTH) has red apiculi and purple stigmas. Genetic analysis showed that red apiculus and purple stigma in LTH co-segregated indicating control by a single dominant gene, or by two completely linked genes. Using 1,851 recessive individuals from two F2 populations, the target gene OsC was delimited to a 70.8 kb interval on chromosome 6 that contains the rice homologue of the maize anthocyanin regulatory gene C1. When the entire OsC gene and its full-length cDNA cloned from LTH were transformed into japonica cultivar Kitaake with colorless apiculi and stigmas all positive transformants had red apiculi but non-colored stigmas, validating that OsC alone was responsible for the apiculus color and represented the functional C gene. OsC was constitutively expressed in all tissues examined, with strongest expression in leaf blades. These results set a foundation to clarify the regulatory mechanisms of OsC in the anthocyanin biosynthetic pathway.
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KEYWORD
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Gene expression, Map-based cloning, OsC, Red apiculus, Rice
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